Effects of Grape Seeds Oil on the Antioxidants of Alloxan Induced Diabetes in Rats

Abstract

This study was examined the effect of grape seed oil on glutathione, malondialdehyde, superoxide dismutase, ceruloplasmin and peroxynitrite radical in normal and alloxan-induced diabetic albino rat males (100 mg/kg body weight). Twenty four rats (12-14) weeks age were selected and randomly divided into four groups each with six replicates,These are: The normal control group, Diabetic group, Diabetic group and treated with grape seed oil and a healthy group receiving grape seed oil. The results of study showed that in the experimentally induced diabetes mellitus rats, oxidative stress caused a significant increase (P<0.05) in malondialdehyde and peroxynitrite radical levels compared with normal control group,while it caused a significantly decrease of glutathione and ceruloplasmin concentrations compared with the normal control. The treatment of alloxan-induced diabetic rats by grape seed oil administration revealed significant decrease in malondialdehyde and peroxynitrite radical levels in comparison with the diabetic control group, while the treatment with grape seed oil caused a significant increase in glutathione and ceruloplasmin. Treating the normal albino rats by grape seed oil showed significant increase (P<0.05) of glutathione and ceruloplasmin concentration and significant decrease (P<0.05) of malondialdehyde and peroxynitrite radical concentration compared with the normal control group. This study conclude that grape seed oil play an important role in treating diabetes mellitus complications and removal of the free radicals and elevating antioxidant levels.