Evaluation of alpha- amylase activity in serum and saliva of normal individuals and patients with jaundice

Authors

  • Hazim H. Eddan Al- Mustansseria University- College of Medicine
  • Jinan F. AI Dahhan Tikrit University - College of Medicine
  • Wasan N. Al- Assee Tikrit University - College of Education
  • Barak F. Sulaiman General Mousl Hospita

DOI:

https://doi.org/10.25130/tjphs.2005.1.6.50.57

Abstract

Enzymes are very important components in the biological system. a-Amylase is one of these enzymes, it is one of the hydrolytic enzymes that breaks down the dietary starch and glycogen to glucose and maltose. The present study is conducted to evaluate a-Amylase activity in serum and saliva of patients with jaundice in comparison it with the enzyme activity in the serum and saliva of healthy individuals. That is, to provide further information about the relationship between jaundice and a­Amylase activity. Estimation of a-Amylase activity in serum and saliva was made by
Amyloclastic (iodometric) method.
A 120 samples of serum and saliva were collected; 50 samples represented the control group whose ages range between (20-55) years and 70 samples were the patient with jaundice group whose ages range between ( 15-55) years. Alpha-amylase activity appeared significantly higher in normal individuals (both in serum and saliva in comparison with patients with jaundice.

References

- Rodwell V. W. and Kennelly P. J. Enzymes: General properties, In: Murry R. K., Granner D. K., Mayes POA and Rodwell VW. Hrper's Biochemistry. 25th ed. Appleton & Lange, California. 2000; 74-85

- Stryer L. Enzymes: Basic concepts and kinetics .. · In: Biochemistry.4th ed. W.W. J:reeman and company , NewYork.200;181-206

- Nelson D. L. and Cox M. M. Enzymes: Mechanism, Structure and Regulation. In: Lehninger Principles of Biochemistry _:3rd_ ed.Worth Publisher USk 2000; 243

- Halkerston I. D. K. Bio­chemistry. 2nd .ed. JOHN Wiley &Sons, NewYork.1988;147.

- Smith A.-f., Beckett G. F., Walker SW and Rae P. Plasma Enzymes Tests in diagnosis In: Lecture Notes on Clinical Biochemistry 6111ed. Blackwell Science, U.K.1998;101

- Champe P. C. and Harvey R A. Lippincott's Illustrated Review: iochemistry. 211d ed. Lippincott Company, Canada. 1994;48 .

- Franco 0. L., Rigden D. J., Melo F. R., Jr C. B., Silva C. P. and Grossidesa M. F. Activity of Wheat alpha-amylase inhibitors to wards bruchid alpha-amylase and structureal explanation of observed specificities. Euir. J. Biochem. 2000; 267: 2166- 2173.

- Linden A. Mayans 0., Klaucke W. M., Antranikian G. and Wilmanns M. Differencial Regulation of a hyperthermophlic alpha-amylase with a Nova! (Ca, Zn) Two metal center by zinc. J. Biol.Chem. 2003; 278(11 ): 9875-9884.

- Koukiekolo R.,Berre-Autn,V. L., Desseaux V.,Moreau Y., Rouge P. Marchi-Mouren G and Sautimone M. Mechanism of porcine Pancreatic alpha-amylase.

Eur.j .Biochem.1999;265 :20-26.

- Hendrickson A. mamalian Pancreatic a-amylase middlebury. dul/CH441A/tutorials/Amy.htm 2002. 11- Moss D. W. and Henderson A. R. Clinical Enzymology. In: Burtis CA and Ashwood E. R. Tietz Textbook of Clinical Chemistry 3rd ed. W.B. Saunders Company. 19999; 689-698. 12- Henery J. B., Davey F. R., Herman CJ, McPherson RA, Pincus MR, Threatte GA and Woods GL. Laboratory diagnosis of Gastrointestinal and Pancreatic Disorders. In: Clinical Diagnosis and management by laboratory Methods W.B.Saunders Company 20th ed. 2001; 462-465.

-Nishide T., Emi M., Nakamura Y.and Matsubara. Correct sequences of cDNAs For Human Salivary and Pancreatic a amylase. Gene. 1986;50:3 71-3 72.

-Wootten I. D. P. and Freeman H.Microanalysis in Medical Biochemistry. 5111ed.Churchill Living­stone. I 982.

-Neillsen J. E., Beier L. , Otzen D., Bouychert T. V., Frantzen H. B., Anderson K. V. and Svendsen A. Electrostatics In The Active Site of a -amylase. Eur.J. Biochem. 1999; 64(3): 16-824.

- Campbell M. K. Biochemistry. ed. Mount Holyoke College, 1999; 442

- Skude G. Pancreatic isoamylase as a routine test . EJIFCC. 2000;12(l)vol.12 No.I

-Moss D. W. and Henderson A. R.Tietz Fundamentals of Clinical Chemistry 4th ed. W.B Saunders Company. 1996;318-323.

- Kaplin L. A. and Pesce A. J. Methds in Clinical Chemistry. Company Universal Mosby , USA. 1987.

- Lorentz K. a -amylase . In : Thomas L. Clinical Laboratory Diagnostics, Use and assessment of Clinical Laboratory Results. 1 sted. T-H book Verlagsgese Schaft Mbh, Germany. 1998;46-51.

- Ben-Aryeh and Cohen M. Salivary Composition in Diabetic Patients. J. Diabetic Complication I 988;2:90-96.

- Haeckel R. Saliva an alternative specimen in Clinical Chemistry . Br. J. Cli. Chem. 1990; 2: 208-217.

- Marshal W. J. The Pancrease In: Clinical Chemistry. 4th ed. Mosby 2000;97-100.

- Finlayson N. D. C. and Bouchier !AD.Disease of the Liver and Biliary system. In : Edward CR, Bouchier IAD, Haslett Cand Chilvers ER.

- Davidson's Principles and Practic of Medicine. 17t11.ed. Churchill Livingstone Company. 1995;498.

- Mayne P. D. The Liver and Gall Stones. In: Clinical Chemistry in Diagnosis and Treatment 6th .ed. Edward Arnold. 1994;498.

- Berk Pdand Wolkoff AW. Bilirubin -Metabolism and the Hyperbilirubinemia. In: Harrison's Principles in Internal Medicine.

th ed. McGraw-Hill Co.2001.

- Gradner H. J. A. Note on The History of Epidemic in Germany. Am. J.Med. 1950;8:587.

-Findlay G. M. and Martin N. H. Jaundice Following Immunization . Lancet. 1973; 1 : 678 -680.

- gate Encyclopedia of medicine Amylase Tests . Gate group 2002

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Published

2023-03-25

How to Cite

H. Eddan, H., F. AI Dahhan, J., N. Al- Assee, W., & F. Sulaiman, B. (2023). Evaluation of alpha- amylase activity in serum and saliva of normal individuals and patients with jaundice. Tikrit Journal of Pharmaceutical Sciences, 1(1), 50–57. https://doi.org/10.25130/tjphs.2005.1.6.50.57