In Vitro new experimentally culture media for Leishmania species cultivation

Abstract

A fifty four different biphasic media were used to cultivate promastigote parasite of Leishmania tropica and compared with ordinary NNN medium, to find a cow milk agar medium can provide a good cultivation to the parasite as well as the ordinary medium, where milk is a cheap and simple, and a good source of lipids, protein, carbohydrate, and calcium. Also, using a 20% of human urine, can enhance the growth rate of parasite, this reduced the time that needed to cultivate the parasite in the ordinary NNN medium. So, using of a modified NNN medium (medium that used the solid phase of ordinary NNN and 20% of human urine as liquid phase) in the field of laboratory can provide a short duration to cultivate Leishmania parasite. The count of Leishmiania tropica, promastigotes ,taken from NNN medium reached, 3 × 106/ml at the end of the 6th day in our new medium, while in NNN medium the number of organisms reached only 1× 106 / ml. also 9.7×107 promastigotes found after the fourteen days of cultivation in media supplemented with urine and 22×106promaastigotes in the tenth days in media with milk. After several passages, the cultured medium ,prepared was .evaluated as, being quite simple, inexpensive, and successful ,compared with, other commercially ,available culture media.