Polymorphism studying of the Angiotensin-converting gene for a group of patients with myocardial infarction in Saladin Governorate

Abstract

Blood samples were collected from 120 people, including 70 people affected by Myocardial Infarction (MI) and 50 healthy people aged between 40 - 70 years. Patients were diagnosed by basis of clinical symptoms and after made laboratory tests in addition to electricity cardio Gram (ECG) by specialists. Also it is measured The concentration of homocysteine for both of groups, where its concentration Patient groups was (45.26 ± 0.71) μmol / L compared to the healthy group (7.86 ± 1) μmol / L. There was a significant difference obviously at level (0.01 and 0.05) between the two groups beside, a mutation Ins / Del was found. Using PCR technique, where appeared three genotypes (II, ID and DD), there was a clear difference in the value of the Allele frequency for Allele D in patients group compared to healthy, where was in patients group(0.664) while in healthy group (0.37). On the contrary, value of the Allele frequency has decreased for Allele I to patients where was(0.336) compared with healthy(0.63) This indicates the association of the deletion mutation (D) with MI and heights level of concentration Homocysteine in plasma. After analysis of Chi square 2, the resent studying proved that society of patients applies Hardy-Weinberg law for balance on it at a significant level (0.01) and Hardy-Weinberg law (p2 + 2pq + q2 = 1) where value of Chi square (7.61)which was less than its tabular value at a significant level (0.01) (9.21). As for the healthy society, it was a balance and applied Hardy-Weinberg law on it at a significant level (0.01) and (0.05) where value of Chi square (5.867) which was smaller than its tabular value at tow levels (9.21) and (5.99).