Detection of mec A gene in Staphylococcus aureus isolated from wounds infections

Abstract

This study aimed to detect the occurrence of mecA gene in S.aureus isolates by PCR technique. The study included a total of 25 S. aureus was collected from patients with a wound infection. Identification of S. aureus was based on growth on manitol salt agar, catalase test, DNase test and coagulase test. Antibiotic Susceptibility test by disc diffusion method was done for all S. aureus isolates against four β- lactam antibiotics and the resistance percentage was as the following: 92% for methicillin, 32% for oxacillin, 60% for amoxicillin –clavulanic acid and 24% for ampicillin – sulbactam. PCR technique was used for the detection of mecA gene in S. aureus isolates and results showed that 24% of isolates were mecA positive, while 76% of the isolates were mecA negative