Induction of brain lesions experimentally by inoculation of Staphylococcus aureus subcutaneously in rabbits (A histological study)

Abstract

Six adult rabbits of both sexes were used tostudy the effect of Staphylococcus aureus on the brain tissue. Animals were classified into two groups. Group(A) was the control and Group(B) was the treated. Group (A) was inoculated with 0.8ml saline solution subcutaneously, and group (B) was inoculated subcutaneously with 0.8m1 suspension of Staph.aureus .The inculation process was repeated again with group (B) after 15 day. After (15) days from the second inoculation, the all animals of both groups were sacrified. A brain specimens from the frontel, parietal and temporal lobes of both groups were taken, processed for histological technique, embedded in the paraffin wax, sectioned at (5) µm thickness and stained by the haematoxylin & eosin for examination by the light microscopy.
The brain tissues of group B, demonstrated the histopathological changes of the whole sites of the brain lobes, included, focal necrosis of the nerve cells, demyelination of the nerve fibers and gliosis. This effect may reflect the efficacy of the bacteria or its toxin or many both on the brain tissues.