Isolation and identification of gram negative bacteria fromYouncl infection in general lrospital at Diyala city

Abstract

Fifty patients with post operative wound infections at a district general hospital in Baaquba were tested for bacterial growth. These patients were exposed to different operations such as (labroectomy,surgical ppendectomy, bullets accidents ,bombing acc.dents anddifferent surgical operations).All patients wounds were swabbed in period from May to August 2006and cultured , the isolated microorganisms were identified on the bases of their morphological ,cultural and biochemical characteristics.twenty six (52%) were positive for bacterial growth .Twenty (77%) yielded a pure culture and six(23%)of mixed culture isolate. These isolates,1(5%)were Pseudomonas aeruginosa 4(20%) were E coli , 3(15%)were Klebsiella pneumoniae and12(60%)were Gram positive. The incidence of bacterial growth was high among adult more than 15 years than children less than 13 years Single and mixed culture were sixty six percent and sixty percent were in adults more than 15 years respectively whereas thirty four and forty percent of single and mixed culture were investigated in children less than 13 years respectively. The study also investigated the bacterial contamination in theater and coach of the same hospital, (1)were Pseudomonas aeruginosa , (3)were E .coli, (0) were Klebsiella and (7) were Gram positive for theater while(0) were Pseudomonas aeruginosa , (2) were E. coli, (1) were Klebsiella and (3) were Gram positive for coaches.